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It achieves this by inhibiting the activity of Acetyl-CoA carboxylase, an essential enzyme in the fatty acid synthesis pathway
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MEPS-HPLC-MS/MS targeted quantitative metabolic profiling of NPs cultures Targeted quantitative analysis of metabolites in NP cultures was performed as previously described [37], using high-performance liquid chromatography coupled to tandem mass spectrometry (HPLC-MS/MS) and a semi-automated microextraction by packed sorbent (MEPS) protocol for the absolute quantitation of selected metabolites: citrate, aconitate, 2-oxoglutarate, succinate, fumarate, malate, oxaloacetate, NADH, NAD + , ATP, ADP, N-acetylaspartate, glutamine, glutamate, aspartate, pyruvate, alanine, valine, leucine, isoleucine
At a DSIP concentration of 1mg/mL, each unit on the syringe delivers 10g of peptide
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