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10.4158/ep-2018-0524 [EL 2

[0038] Add 2-aminoisobutyric acid (1.24g, 12.0mmol), triethylamine (1.21g, 12.0mmol), chloroform (20mL) into the reaction flask, lower to 0C, add the above compound III (4.32g, 10.0 mmol) was incubated for reaction, and after TLC detected that the reaction was complete, water (20 mL) was added to the reaction system and stirred for 10 minutes, the organic phase was collected, and concentrated under reduced pressure until no liquid flowed out

It is commonly used in cellular assays, receptor activation studies, and metabolic models to assess endocrine communication and regulatory feedback mechanisms