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Slice electrophysiology Immediately following acute electronic vapor exposure (PG/VG N = 6, Nic N = 6) or the last session of repeated electronic vapor exposure (PG/VG N = 6, Nic N = 6), mice were rapidly decapitated, and brains were extracted and placed into an ice-cold sucrose solution containing the following: 206.0 mm sucrose, 2.5 mm KCl, 0.5 mm CaCl 2 , 7.0 mm MgCl 2 , 1.2 mm NaH 2 PO 4 , 26 mm NaHCO 3 , 5.0 mm glucose, and 5 mm HEPES