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Unhealthy lifestyle, medications, infections, chronic stress and exposure to chemicals and toxins lead to excessive production of free radicals, and cause your body to use more glutathione (and also other antioxidants) to neutralize free radicals and limit the oxidative damage

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For instance, amplification of the CCNE1 gene is closely associated with resistance to PARP inhibitors and platinum-based agents (99)

Most of these studies enrolled limited series of patients