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Materials and methods Cell culture MCF-7(#HTB-22), ZR-75.1 (#CRL-1500), and MDA-MB-231 (#HTB-26) cells obtained from American Type Culture Collection (ATCC, LGC Standards, Molsheim, France) were maintained in DMEM medium (Dulbecco modified Eagles medium) supplemented with 10% ( v/v ) FBS (fetal bovine serum)

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