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Fatalities related to MC exposure were also recorded

The cell lysate was clarified at 13,400 g for 10 min at 4 C and the supernatant was subjected to a Cu(I)-assisted click reaction with biotin picolyl azide followed by loading onto a 10 kDa spin column to remove the free biotin picolyl azide using lysis buffer

Its efficacy in lowering blood sugar levels and contributing to weight loss has been widely acknowledged