GST Tag Interference with Protein Function Challenge : In some cases, the GST tag can interfere with the function of the target protein, particularly if the protein's active site or interaction domains are close to the fusion point
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Western blot BF muscle from both the acute (intraoperative) and chronic (1 week) oIGF-1 infusion groups was homogenized in lysis buffer (Cell Signaling, Danvers, MA) with protease and phosphatase inhibitors (Sigma-Aldrich, St

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