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After the screening, baseline characteristics (including age, gender, body mass index (BMI), and cirrhosis duration) and the frequency, duration, and severity of cramps before and after L-carnitine therapy were gathered in a form

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Reverse transcription and real-time quantitative PCR were performed according to the instructions provided with the Evo M-MLV RT Kit with gDNA Clean for qPCR II (AG11728, AG) and PerfectStart Green qPCR SuperMix Kit (AQ601-02, TransGen Biotech)