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Briefly, 100 L of CuCl 2 (1 M, 13.445 mg) solution was added to 10 mL of BSA (10 g L 1 ) solution and stirred at room temperature (RT, 25 C) for 5 min
Liver Detoxification Glutathione binds to toxins, heavy metals, and metabolic waste, converting them into water-soluble compounds the body can safely eliminate through bile and urine
After the cells were counted, they were seeded into 125 ml or 500 ml cell culture Erlenmeyer flasks (Corning) at a bulk cell density of 3.5-4 ml of media/million cells, (see 9 ) in Essential 8 (Life Technologies) with 10 M Y-27632 (BOC Sciences)
