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Briefly, approximately 1.510 5 host cells (A549, RAW 264.7, PBL-985, and PBL-985 nox2 -KO cell lines) were seeded per well in 12-well plates and cultured in DMEM + 5% FBS at 37C and 5% CO2 for 24 h until reaching 95% confluence, equivalent to approximately 3 10 5 cells

Each laboratory is responsible for ensuring that all local regulations, institutional policies, and safety guidelines are followed when handling these materials

It also aids in reducing inflammation and enhancing immune system function

Each session lasts approximately 3045 minutes and is pain-free, with patients often reporting visible improvements after just a few treatments