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It mimics the gut hormone GLP-1 to: slow stomach emptying (you feel full sooner) stimulate insulin release and suppress glucagon (better blood-sugar control) act on appetite centers in the brain (reduced cravings) Animal and early human studies suggest these mechanisms kick in below the 1 mg weekly dose, as discussed in Healthlines explainer on microdosing

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Because those signals converge on appetite from different directions, the hypothesis is greater total reduction in energy intake than either alone

The digested tissue was centrifuged to remove debris, resuspended in DMEM, and filtered through a 100-m nylon mesh to obtain a single-cell suspension