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CFU immune cells cluster assay Immune cells freshly isolated from the kidney marrow of young-adult (8-week-old) and aged (17-week-old) fish were resuspended in Roswell Park Memorial Institute (RPMI) 10% medium (RPMI + HEPES 1:40 + heat-inactivated FCS 10% + 1:500 diluted solution containing gentamicin 5 mg ml 1 and amphotericin B 125 g ml 1 ) and seeded at a concentration of 200,000 cells per well in the wells of a 96-well plate