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The binding reaction was then neutralized with a buffered pH change, and the peptide-HLA complexes were transferred to a 96-well plate coated with a pan-HLA-DR antibody, clone L243 (BioLegend, San Diego, CA, United States), and incubated overnight

An HPLC result does not by itself establish complete identity, total peptide quantity, sterility, endotoxin status, absence of every contaminant, full-life stability, safety, efficacy, regulatory approval or pharmaceutical equivalence